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Turkish Journal of Cancer
2001, Volume 31, Number 1, Page(s) 21-26
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Comparative genomic hybridization of primary skeletal Ewing's sarcoma
İBRAHİM KESER1, ELISABETH BURCKHARDT2; NURDAN TUNALI3, MUALLA ALKAN2
1Department of Medical Genetics, Akdeniz University Medical School, Antalya- Turkey
2Department of Cytogenetics, Institute of Pathology, University of Bern, Bern-Switzerland
3Department of Pathology, Çukurova University Medical School, Adana-Turkey

We used comparative genomic hybridization (CGH) technique to identify the genetic changes in 8 primary skeletal Ewing's sarcoma samples from archieved paraffin-embedded tissues from children (4-17 years old) using double step degenerate oligonucleotide-primed polymerase chain reaction (DOP-PCR). CGH analysis showed that the most common genetic alterations were losses of chromosomes 1p, 2q, 3p, 14q, 21, X and gains of chromosomes 8q and 17q. Chromosomal deletions were found only in samples taken from males, while amplifications were identified in both sexes. As a result, this difference may explain why tumor occurs about 1.5 times more commonly in males than in females.

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