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Turkish Journal of Cancer
2005, Volume 35, Number 2, Page(s) 088-092
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Expression of multi drug resistance (MDR1) gene in human promyelocytic leukemia cell line selected with vincristine
YUSUF BARAN1, UFUK GÜNDÜZ1, ALİ UĞUR URAL2
1Middle East Technical University, Department of Biological Sciences, Ankara
2Gülhane Military Medical Academy, Department of Hematology, Ankara-Turkey

MDR1 mediated drug resistance is one of the main problems for some cancer chemotherapies. Vincristine (VCR) is an effective drug used in clinical treatment of acute leukemias. The concentration of VCR was stepwise increased (1-50 nM) in the cell culture media of HL60 cells (AML cell line). The viability of cells was determined and the expression of MDR1 gene was studied by using reverse transcriptasepolymerase chain reaction (RT-PCR). In sensitive HL60 cells MDR1 gene expression was not present. The level of the MDR1 gene expression increased in cells with stepwise increases of VCR concentration. MDR1 gene expression was about six times more in 50 nM VCR resistant cells than in 2 nM VCR applied HL60 cells. The IC50 values in sensitive and resistant HL60 cells were 4 nM and 300 nM, respectively. The results indicated that HL60/VCR cells were 75-fold more resistant to vincristine compared to sensitive HL60 cells. It was observed that the cells that were resistant to high concentrations of VCR express MDR1 gene at high levels.[Turk J Cancer 2005;35(2):88-92].

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